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The percent (%) cytotoxicity was determined using the following formula for each given experimental treatment: Cytotoxicity (%) = 100 [(Experimental Culture Medium Background)/(Maximum LDH Release Culture Medium Background)]
Neurobiol Aging 26(1):115123
For instance, in mouse embryonic stem cells, targeted disruption of glucose-6-phosphate dehydrogenase, which is the rate-limiting enzyme in the pentose phosphate pathway, the predominant metabolic pathway for the generation of NADPH (a cofactor of GR), renders cells extremely sensitive to H 2 O 2 -induced cell death 34
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