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cDNA was synthesized and then quantitative PCR (qPCR) amplification was conducted with the C1000 Thermal Cycler (Bio-Rad, catalog CFX384) using iQ SYBR Green Supermix (Bio-Rad)

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If either you or your partner were born with a cleft lip or palate, your chance of having a baby with a cleft is also around 2 to 8%

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