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Protein purification Harvested bacterial cells were lysed on ice for 60 min by treatment with lysozyme (0.2 mg ml 1 ) and Protease Inhibitor Cocktail Tablets, EDTA-Free (Sigma-Aldrich) followed by sonication 5X 20 s and centrifuged at 30,000 g for 30 min

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When Marienne arrives, Love invites her inside for an adult conversation

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