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The BsNb PV2-PV3 constructed by simply fusing two nanobodies PV2 and PV3 (BsNb-fusion) was also characterized for comparison

Different goals may benefit from different dosing approaches

For the HEDS assays, either GSH was varied between 100 M and 1.5 mM for ScGrx (or 250 M and 3.0 mM for PfGrx) at fixed concentrations of HEDS (0.18, 0.37, 0.55 and 0.74 mM) or HEDS was varied between 90 and 920 M at fixed concentrations of GSH (0.3, 0.5, 1 and 1.5 mM for ScGrx7 or 0.25, 0.5, 1 and 2 mM for PfGrx)

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[12] Ghrelin receptors are known for their role in controlling appetite, and their activation may lead to heightened hunger cues, potentially resulting in increased body mass