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For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

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Important safety considerations: Patients should contact their GP if they experience severe injection site reactions, signs of infection (fever, spreading erythema), chest pain, palpitations, or allergic symptoms

Freezing can denature the peptide fragments and reduce biological activity

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