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Bacteriostatic water is usable for 28 days after opening due to its benzyl alcohol preservative

No published data exists for treatment periods extending beyond six months

If they are not, stay put or drop back

Freeze-thaw cycling is especially destructive to peptides each cycle forms ice crystals that physically shear the molecule, so even one accidental freeze can tank a vial

Signs that you may be deficient in B12 may include (but are not limited to): weakness, tiredness, or light headedness, heart palpitations and shortness of breath, nerve problems like numbness or tingling, muscle weakness and loss of appetite/weight loss

Baltaci, Davut, et al