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Abbreviations MDA: Malondialdehyde ROS: Reactive oxygen species OS: Oxidative stress GSH: Glutathione CKD: Chronic kidney disease CRF: Chronic renal failure ECM: Extracellular matrix STING: Stimulator of interferon genes mtDNA: Mitochondrial DNA ATP: Adenosine triphosphate HE: Hematoxylin and eosin PAS: Periodic acidSchiff cGAS: Cyclic GMPAMP synthase MTT: Methylthiazolyltetrazolium TUNEL: Terminal deoxynucleotidyl transferase dUTP nick-end labeling SOD: Superoxide dismutase SCR: Serum creatinine UPR: Urinary protein IL: Interleukin TNF-: Tumor necrosis factor- Hyp: Hydroxyproline MMP: Matrix metalloproteinase LC3B: Microtubule-associated proteins 1A/1B light chain 3B Col-1: Collagen I -SMA: -Smooth muscle actin FN: Fibronectin ATG5: Autophagy-related 5 p62: Sequestosome 1 TBK1: TANK-binding kinase 1 IRF3: Interferon regulatory factor 3 BCL-2: B cell lymphoma-2 BAX: BCL-2-associated X protein GFR: Glomerular filtration rate dsDNA: Double-stranded DNA cGAMP: Cyclic GMPAMP DAMPs: Damage-associated molecular patterns References Ahmed A, Trezza A, Gentile M, Paccagnini E, Lupetti P, Spiga O et al (2022) The drp-1-mediated mitochondrial fission inhibitor mdivi-1 impacts the function of ion channels and pathways underpinning vascular smooth muscle tone

Circadian Rhythms and Antioxidant Defense Timing Glutathione metabolism follows circadian patterns, with peak synthesis occurring in early morning and declining through evening