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This is accomplished by mixing NHS with a carboxyl-containing molecule and the carbodiimide EDC (Cat

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Mishandling your diluent is a fast track to compromising incredibly valuable research materials and, even worse, invalidating your data

All the other solvents and reagents were used as received without further purification

Vigorous agitation can denature the peptide

Glutathione is a required substrate for a family of enzymes called glutathione S-transferases, which conjugate glutathione to reactive compounds including drug metabolites and environmental toxins making them water-soluble for excretion