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C2C12 mouse myoblasts are widely used for studying IGF-1 LR3's effects on myogenic differentiation, myotube hypertrophy, and Akt/mTOR-mediated protein synthesis signaling

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Animal studies have also found that intermittent fasting lowers IGF-1 more effectively than long-term calorie restriction, but other studies have found no difference between the two (32, 33)

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