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The enriched cells were directly analyzed by flow cytometry to determine the positive cell ratio or subjected to RNA preparation and RT-qPCR analysis to determine the enrichment fold with GST RNA expression
PMID 10496658)Tier 1 primary [3]Beck et al
This inhibition causes AICAR to accumulate intracellularly
Moreover, amino acid metabolism regulates the redox balance through the generation of nicotinamide adenine dinucleotide phosphate (NADPH) and reduced glutathione (GSH), which are essential for cellular defense against oxidative stressa key driver in IPF progression [7, 19]