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Detecting or confirming direct interactions between proteins or protein complexes

Male (n=30) who are 31.175.02 (MeanSD) and have sperm concentration15 million/mL has been defined as normozoospermia group

Lipids were extracted using ice-cold methanol, isopropanol and water (45:45:10) containing 0.3 M KOH, followed by saponification (60 C, 1 h)

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Our previous studies, using in vitro cell culture as well as in vivo animal models have also demonstrated that enzymes cytochrome c oxidase (Complex IV) and aconitase are extremely sensitive to mitochondrial-ROS induced oxidative stress in comparison to other complexes [23],[38],[42],[43]