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Visual analysis was conducted in a double-blinded manner to ensure unbiased assessment

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Up to now, there have been numerous pharmacological studies on the treatment of IS by BC and its active components

These are easily avoided by holding direct pressure after removal of the IV

Digestion-based DNA methylation analysis Percentage DNA methylation was assessed at CpG sites across CpG islands in CYP2R1, CYP27A1, CYP27B1, CYP24A1, and VDR genes using Qiagen EpiTect II methylation enzyme kits and PCR assays at the concentrations and cycle conditions recommended by the manufacturer

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