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RNA extraction, quantitative PCR Total RNA was prepared using Tri Reagent (Sigma-Aldrich, Saint Louis, Missouri, USA), agarose gel checked for integrity, and reverse transcribed with cDNA synthesis kit (BioRad, Milano, Italy) according to the manufacturers instructions
The target pathway under investigation direct lipolysis, receptor-mediated signaling, or upstream hormone stimulation is typically the deciding factor in which compound class fits a given research design
Some do a short loading phase, then move to maintenance
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