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This meta-analysis has several limitations
Busetto GM, Agarwal A, Virmani A, Antonini G, Ragonesi G, Del Giudice F et al
In addition, RAM contains particular cell types, as well as extracellular matrix molecules which are vitally distinct from those found in healthy sites, including inflammatory factors, reactive oxygen and nitrogen species (RONS), immunogenic proteins, and oxygen [8, 9]
More importantly, since GST is highly expressed protein with excellent solubility, fusing a difficult to express protein to the GST tag sometimes can greatly enhance the recombinant protein's expression as well as it solubility
The blood extract was analyzed as such and whole blood sample was treated with methanol to precipitate proteins followed by liquid-liquid extraction in chloroform
The most noticeable difference I observe in my patients is the shortening of the recovery time
