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Beads were washed with 20 mM and 40 mM imidazole steps and proteins were eluted using 250 mM imidazole in 50 mM Tris-HCl, 100 mM NaCl, 0.5 mM MgCl 2 (pH 7.5)

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Sin embargo, siempre es recomendable consultar a tu mdico antes de iniciar cualquier suplemento, especialmente si tienes condiciones mdicas preexistentes o ests tomando medicamentos