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The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board

L-Carnitine is a very small molecule, soluble in water, naturally found and highly concentrated in muscles (greater than 95% of total carnitine)

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