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For the western blotting of HMGB1, frozen cortices were homogenized in 50 mM TrisHCl (pH 7.5) containing protease inhibitor cocktail and phosphatase inhibitor cocktail

Of the eight glutaredoxins in Saccharomyces cerevisiae 11,12 , ScGrx6 and ScGrx7 are the only monothiol isoforms that are active in standard assays 11,12,18,22,23,24

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