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There was a 1.4-fold decrease for HEK-293 cells and a 1.1-fold decrease for K-562 cells in total glutathione levels at 25M of menadione compared to the vehicle control

And while the official guidance says they are all equivalent, the real-world experience of thousands of researchers tells a more nuanced story

Considering a 20% dropout rate, the sample size will be inflated to 250 (approximately 63 per group) is required

Drakos E, Atsaves V, Li J, Leventaki V, Andreeff M , Medeiros LJ, Rassidakis GZ

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Dehydration worsens side effects, particularly nausea, fatigue, and constipation