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Human antimicrobial peptide LL-37 induces glial-mediated neuroinflammation

Samples were reduced with DTT and alkylated with iodoacetamide as described above, then diluted with DPBS (300 L), and taken forward to LysC and trypsin digestion without precipitation

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This method has gained popularity due to its higher bioavailability compared to traditional oral supplements

Furthermore, MD revealed decreased flexibility of p53 TAD2 in complex with FOXO4-DRI compared to the FOXO4 FH -bound state with RMSDs of 1 heteronuclear NOE data was recorded using the HSQCNOEF3GPSI bruker pulse sequence (32 scans, 256 points in F1, 1024 points in F2, 1278.772 Hz spectral width in F1, 9615.385 Hz spectral width in F2) using a three seconds saturation period