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CRISPR-Cas9-mediated gene knockout Ythdf1 , Ythdf2 , Ythdf3 , and Mettl3 knockout (KO) EpiSC cells were generated using the lentivirus-mediated CRISPR-Cas9 technology

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Cellular studies have shown that exogenous NAD + can impact mitochondrial activity, such as boosting Krebs cycle and the ETC, but also impair genomic DNA replication and induce DNA replication stress due to impairment of pyrimidine biosynthesis 118

For the full protocol breakdown, see our guide to the Wolverine peptide stack

It is a powerful antioxidant that plays a role in many biochemical processes

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