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Agar gels were mixed with 1 mL PBS by vigorous pipetting before the addition of calcein-AM, DCFDA, or mBcl to the same concentration as indicated for cells cultured in liquid medium and incubated for 30 min in the dark at the temperature indicated for those cells
Finally, Hassan et al.investigated the protective effects of carvedilol against hepatic ischemia-reperfusion injury in rats

Round up for testing losses, damaged supplies, spills, and changes to the research plan
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Resuspended cells were homogenized in a Dounce homogenizer for 3050 times on ice and spun at 700 g for 5 min at 4 C

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