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Nature , 458 (7239), 7625
How do protein needs increase during preconception and pregnancy Research shows that women need more protein during preconception and pregnancy than previously thought
Positive clones were selected for by puromycin treatment, single-cell sorting, cell expansion, and confirmation by gene amplification and sequencing for correct gene cassette insertion, and immunoblotting for expression of the Cas9 protein after doxycycline treatment
LC-MS/MS parameters The chromatographic separation was achieved using a Biphenyl (Kinetex, 3 x 150 mm, 2.6 m) column with the mobile phase 0.1% formic acid:methanol (30:70) at 0.5 mL/min
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