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Samples were eluted with isocratic flow 2/98 (acetonitrile/ammonium formate, pH 2.5) for 2 min, followed by gradient from 2/98 to 70/30 in 1 min, then isocratic mobile phase 70/30 (acetonitrile/ammonium formate, pH 2.5) for 2 min, and a final gradient from 70/30 to 2/98 (acetonitrile/ammonium formate, pH 2.5) for 3 min

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Yes, some individuals experience mild abdominal cramping, bloating, or discomfort when taking vitamin B12 supplements, particularly on an empty stomach or with higher-dose formulations

Therefore, (hobby) weightlifting may not negatively affect IVDs chronically or acutely

Experimental models have shown that DSIP administration increases GABAergic activity while reducing excitatory neurotransmission in cortical and limbic regions