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The ShapiroWilk test determined that the data were normally distributed

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Its effect is local, confined to the patch of skin you treat

Hayot M, Michaud A, Koechlin C, Caron MA, LeBlanc P, Prefaut C, Maltais F

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The slides were washed in PBS and then blocked using 10% goat serum in PBS for 1 h at room temperature before adding primary antibodies diluted in the blocking buffer