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GSR was inserted behind BBa_K880005 (strong promoter + strong ribosome binding site), then inserted before the 10x his-tag and double terminator

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(2020) published a validated detection assay specific to LongR3-IGF-I, Des(1-3)-IGF-I, and R3-IGF-I in athlete samples

References Minich, D

Metabolic response to a pantothenic acid deficient diet in humans