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The final mixture containing 6.5 M guanidine hydrochloride was heated at 95 C for 15 min
Melanin is what gives skin its color and helps protect us from UV rays
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Furthermore, 20 L serum was mixed with the detection reagent and detected twice, before and after a 5 min water bath
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This review was also limited in that it only included articles available in English and did not access articles behind paywalls, which may have possibly introduced selection or publication bias to the methodology
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