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The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

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Although glutathione is an antioxidant produced naturally by the body, its production may not be sufficient for various reasons

One failed our tests for providing only 81% of its listed amount of glutathione

human efficacy is unproven [3] [4]