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Long-term side risks could consist of the body generating antibodies against the drug, which could reduce its efficacy or cause new side effects

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The supernatant was passed through a mouse anti-human AIM IgG monoclonal antibody (CL7)-immobilized HiTrap NHS-activated HP column and the protein was eluted with 0.1 M glycine-HCl (pH 2.5) and neutralized with 1 M Tris-HCl (pH 8.5)