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that may overlap with one of the peaks of GS-NEM

These changes may increase oxidative stress and infection risk, suggesting caution with vitamin E use in this vulnerable population (Wald et al., 2022)

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usually, this is done through a vein

The samples were subsequently washed with 1 ml of 1% BSA and 0.5% Tween-20 in PBS, centrifuged and the pellets were resuspended in 1 ml of PI solution (20 g ml 1 propidium iodide (Sigma-Aldrich, P4170), 0.5 mg ml 1 RNase A (Sigma-Aldrich, R5503) and 0.1% Triton X-100 in PBS)