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As a result, some patients experience worsening symptoms after switching from PN to PLP [35]
In brief, HUVECs were lysed in the Co-IP buffer containing 20 mM Tris-HCl (pH7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, 0.5% protease inhibitor cocktail on ice for 20 min
Practical Example: A vegan experiencing mild fatigue might find that a daily high-strength oral B12 spray or tablet completely resolves their symptoms
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