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C., Yeh, W
The agitation introduces air bubbles that create foam, and the foam-liquid interfaces generate shear stress that denatures the protein
Zoospores were incubated for 48 h before exposure to either 2 mM GSH or 2 mM GSSG, and zoospore production calculated by counting the next generation of motile zoospores at various time points (24 h 90 h) post glutathione exposure
J Biol Chem 110(3):665679 Ensafi AA, Rezaei B, Nouroozi S (2009) Flow injection spectrofluorimetric determination of cystine and cysteine
Ideally, you want it hot enough that the water in the pot simmers, but does not boil