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For cell immunostaining, HeLa cells were transfected with different plasmids for 12 h and fixed in 4% paraformaldehyde for 1 h at room temperature

This study was designed to investigate the possible synergistic effect of GSH and SeNPs on the post-thawing laboratory indicators of sperm quality, oxidative-antioxidant status, and ability of spermatozoa to support in vitro development

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doi: 10.1016/j.aquatox.2017.12.017 30 HouWYulinHWenlongYTianTPengFYuqiongDet al