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To dissect the upstream regulatory mechanism of miR-29b-3p under Gln deprivation conditions, we integrated transcriptome data (GSE148016) from both Gln+ and Gln- groups, and performed cross-analysis with the predicted upstream transcription factors of miR-29b-3p, ultimately identifying five candidate transcription factors
Therefore, affinity-purified rEE2 eluates and rMCP insoluble fractions were further subjected to size exclusion chromatography (SEC) purification: For rEE2, suitable eluates were further purified using Superdex 200 increase 10/300 or 16/600 columns (Cytiva, Little Chalfont, UK) on a GE AKTA Explorer system (GE Healthcare)
Effective and works well for a great price
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Its about trying to get ahead of it versus react to it
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