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The most profound conformational change is the sharp kink in the middle of TM6, leading to an outward movement of the intracellular portion of TM6 as measured by C carbon of Lys 6.35b (19.0 , similar to that of other G s -coupled class B receptors)

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This test can be performed using an over-the-counter at-home testing kit and is used to confirm a mid-cycle surge in LH release, which suggests the occurrence of ovulatory cycles