After 72 h of methanol induction, the expressed extracellular GSTA2X protein samples were separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by staining with Coomassie Brilliant Blue R-250 and silver staining reagent (Beyotime Biotechnology, Shanghai, China)
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It is catalysed by glutamate-cysteine ligase, historically called gamma-glutamylcysteine synthetase
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Open Field On PND 27 and 28, two hours after the three-chamber test on PND 28, rats were subjected to the open field test to assess exploratory and locomotor activity (Deacon 2006)
Can B12 Cause Anxiety