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To demonstrate whether a specific cytokine plays a role in angiogenesis after hindlimb ischemia, we measured mice serum cytokine levels using Luminex technology in four groups: WT sham ( n = 3), WT HLI ( n = 4), KO sham ( n = 3), and KO HLI ( n = 4) to compare cytokine releasing levels between WT mice and GSDMD -/- mice after HLI (Fig

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The goal is to achieve a steady weight loss of 1 to 2 pounds per week , which is considered a safe and sustainable rate

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