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Individual results vary considerably, but community experience reveals general patterns

The amount of water you add does not change the total peptide in the vial, but it affects how many usable draws you can get

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Procedure: Inject the solvent slowly against the inner wall of the vial rather than directly onto the powder bed

John Wiley and Sons, Inc, Hoboken, NJ (2011) Kalgutkar, A.S., Vaz, A.D., Lame, M.E., Henne, K.R., Soglia, J., Zhao, S.X., Abramov, Y.A., Lombardo, F., Collin, C., Hendsch, Z.S., Hop, C.E.: Bioactivation of the nontricyclic antidepressant nefazodone to a reactive quinone-imine species in human liver microsomes and recombinant cytochrome P450 3A4