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For the immunohistochemical technique, 3 m sections were done of each selected paraffin block, using the Envision System (Dako, USA), following standardized protocol with adequate controls and using the following antibodies: S100 protein, HMB45, Neuron specific enolase (NSE), Cytokeratin AE1-AE3 (CKAE1-AE3), (GFAP), chromogranin, synaptophysin and p53
Supplementary Figs 57), a valuable and well established approach in modelling human neurological disorders in vitro 22,27
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