Materials for Cell Culture The 293 T cells were maintained in Dulbeccos Modified Eagles Medium, low glucose (DMEM-low glucose, Gibco, Thermo Fisher Scientific, #11885084) supplemented with 10% FBS and 1% P/S in a humidified incubator at 37 C with 5% CO
10.1515/znc-1991-9-1025 61 GullnerG.ZechmannB.KnstlerA.KirlyL
For M2 polarization, RAW264.7 cells were stimulated with IL-4 (20 ng mL 1 , ABclonal, China) for 24 h
Preliminary findings from these studies suggest a tolerable oral dosage of approximately 100mg/kg in mice [1]
[PMID: 15218992] PubMed record related to this compound for laboratory literature review
Product background The insulin-like growth factors (IGFs) are a family of proteins with a similar structure to insulin