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Blood 132(Suppl 1), 2018

doi: 10.1111/dom.13023 294 TirucheraiGMoraJRevankarRCharlesE

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After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader

The diversity of these drugs is reflected not only in their therapeutic uses and mechanisms of action but also in their routes of administration and widespread global production, underscoring the biological importance of H 2 S as a gaseous signaling molecule and its broad potential in pharmacological interventions