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Interestingly, a few cases of differential metabolic changes (ie, fold change of MTX treatment over basal levels) were associated with MTX resistance (at 96 hours)

Also, note that DMSO can oxidize sensitive residues like cysteine over time, so you may need to use degassed solvents or add antioxidants if thats a concern

Notably, these deletions are mostly one nucleotide in length, ensuring that nearly the entire sequence, including variant positions, is retained

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Using Too Little Water Adding 0.5mL to a 100mg vial gives 200mg/mL