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Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)
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All methods involving human material were carried out in accordance with the Declaration of Helsinki guidelines and regulations
(2020) 158:19992014.e1
C., Tschume, L
Certain Antidepressants: Especially those affecting serotonin levels, can lead to a serious condition known as serotonin syndrome
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