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J Immunol Methods 219:187193 Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome

The copper ion participates in redox reactions and is thought to facilitate peptide uptake into cells

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After overnight culture, the cells were co-transfected with pGloSensor22F cAMP plasmid (Promega) and GLP-1R DNA using Lipofectamine 2000 transfection reagent (Invitrogen)