HSA was immobilized to the surface by pre-activation (1.0 EDC, 1.0 M NHS in PBS, pH 7.4, 7 min) followed by injection of HSA to flow cell 1 (30 g ml 1 in 10 mM NaOAc buffer, pH 5.0, twice for 7 min)
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[51] Due to the risks associated with folate deficiency, folic acid fortification of foods was initiated
Limitations This consensus reflects the views of a multidisciplinary expert panel in Kuwait but was not based on a formal Delphi process or structured stakeholder engagement, such as patient or caregiver input