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When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)

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(5) L Pickart, J Michelle Vasquez-Soltero, and A Margolina (2015) GHK-Cu may Prevent Oxidative Stress in Skin by Regulating Copper and Modifying Expression of Numerous Antioxidant Genes Cosmetics, July 2015, Volume 2 (Issue 3), Pages 236-247

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Inconsistent application Sporadic use produces sporadic results