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The vessels were continuously superfused with Krebs buffer solution (118 mM NaCl, 4.7 mM KCl, 1.5 mM CaCl, 25 mM NaHCO, 1.1 mM MgSO, 1.2 mM KHPO, and 5.6 mM glucose) at 37 C, gassed with 95% air and 5% CO

Special considerations: children and adolescents Several GLP-1RAs, including liraglutide and dulaglutide, are approved for treating children and adolescents with type 2 diabetes

Why this approval matters While the oral version of Wegovy is new, its not the first oral GLP-1 medication to be approved in the U.S

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After incubation with the appropriate antibodies for 30 min, erythrocytes were lysed with lysis buffer (BD FACS Lysing Solution, BD Biosciences, San Jose, USA) for 10 min on a shaker, followed by centrifugation (250 x g, 5 min, 4C, Centrifuge 5427 R, Eppendorf SE, Hamburg, Germany) and washing with HBS

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