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Cells were first gently lysed with the cytoplasmic extraction buffer to release cytoplasmic components, and the supernatant was collected after centrifugation as the cytoplasmic protein fraction

Diabetes or metabolic syndromes: People living with diabetes or metabolic syndromes, such as high blood pressure, high blood sugar, excess body fat around the waist, and irregular cholesterol levels, may also experience inflammation in the body, which can impact the health of the shoulder and rotator cuff, particularly leading to adhesive capsulitis (frozen shoulder)

Olson K, Dover JS, Arndt KA