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While incubation of the peptides with purified human DPP-4 resulted in rapid degradation of hGLP-1 (50% reduction of intact peptide within 1 hour) the researchers found that both echidna and platypus GLP-1 were not degraded by human DPP-4confirming that monotreme GLP-1 is resistant to DPP-4 cleavage. They did identify that the monotreme GLP-1 peptides were less potent at activating the GLP-1 receptors, and when they did bind, they initiated a different signaling cascade than the human version does

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