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Greenish or brownish discoloration indicates copper oxidation and peptide bond cleavage

At the experimental endpoint, the cells were fixed with 10% trichloroacetic acid (Sigma-Aldrich, T6399-500G) and stained with 0.05% SRB for 1 h and then washed repeatedly with 1% acetic acid

Microbiota profiling analysis showed that some differentially abundant taxa are related to memory

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While fasting (for example, from 12 midnight until 7 am), we assume that the input is (0.25)A

It's not ideal because the IR is a moving target, but it's the best method I have found yet and certainly gets you in the ball park