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Dose consistency: Irregular dosing produces irregular results
Seasonal cycling: Some researchers run one 12-16 week cycle annually, typically in winter when sun exposure is reduced
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As a result, DES(1-3) IGF-1 exhibits roughly 10-fold greater potency than native IGF-1 in cell-based assays, despite identical IGF1R binding affinity the potency difference is entirely attributable to less sequestration